The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 11, 2020

Filed:

Dec. 15, 2016
Applicant:

Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd., Shanghai, CN;

Inventors:

Yaoji Xuan, Shanghai, CN;

Sitian Gu, Shanghai, CN;

Wei Wu, Shanghai, CN;

Sha Liu, Shanghai, CN;

Yueyi Bao, Shanghai, CN;

Qiwen Niu, Shanghai, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/48 (2006.01); C12N 15/00 (2006.01); C12N 1/20 (2006.01); C12P 7/00 (2006.01); C07H 21/04 (2006.01); C12N 9/16 (2006.01); C12N 15/63 (2006.01); C11B 3/00 (2006.01); C12N 9/20 (2006.01); C12R 1/085 (2006.01);
U.S. Cl.
CPC ...
C12N 9/16 (2013.01); C11B 3/00 (2013.01); C11B 3/003 (2013.01); C12N 9/20 (2013.01); C12N 15/63 (2013.01); C12P 7/00 (2013.01); C12Y 301/04003 (2013.01); C12R 1/085 (2013.01);
Abstract

Provided is a mutant of the wild type phosphatidylcholine-specific phospholipase C of. The mutations involved comprise the amino acid residue at position 63 being mutated from asparagine to aspartic acid, the amino acid residue at position 131 being mutated from asparagine to serine, and the amino acid residue at position 134 being mutated from asparagine to aspartic acid, and may comprise the amino acid residue at position 56 being mutated from tyrosine to alanine, lysine, asparagine, glutamine, histidine or tryptophan, and further, may also comprise the amino acid residue at position 106 being mutated from methionine to valine. Also provided are a polynucleotide molecule encoding the mutant, a nucleic acid construct and a host cell comprising the polynucleotide molecule, a composition comprising the mutant, and the use of the mutant, the polynucleotide molecule, the nucleic acid construct and the host cell.


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