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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10736920 B1

PDFFull Text
Date of Patent:
Aug. 11, 2020

Filed:

Nov. 13, 2017

Pdl1 block car-t transgenic vector for suppressing immune escape, preparation method thereof, and application of the same

Applicant:

Shanghai Unicar-therapy Bio-medicine Technology Co., Ltd, Shanghai, CN;

Inventors:

Lei Yu, Shanghai, CN;

Liqing Kang, Shanghai, CN;

Zhou Yu, Shanghai, CN;

Assignee:

SHANGHAI UNICAR-THERAPY BIO-MEDICINE TECHNOLOGY CO., LTD, Shanghai, CN;

Attorney:

Bayramoglu Law Offices

Primary Examiner:

Shin Lin Chen

Int. Cl.
CPC ...
A61K 39/21 (2006.01); C07H 21/04 (2006.01); C12N 15/00 (2006.01); C12N 15/63 (2006.01); A61K 35/17 (2015.01); A61K 39/395 (2006.01); C07K 16/28 (2006.01); C12N 15/86 (2006.01); C12N 15/867 (2006.01); A61K 48/00 (2006.01); A61P 35/00 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
A61K 35/17 (2013.01); A61K 39/395 (2013.01); A61K 48/00 (2013.01); A61P 35/00 (2018.01); C07K 16/2818 (2013.01); C07K 16/2827 (2013.01); C12N 15/86 (2013.01); C12N 15/867 (2013.01); C07H 21/04 (2013.01); C07K 2317/24 (2013.01); C12N 2015/8518 (2013.01); C12N 2820/55 (2013.01); C12N 2820/60 (2013.01); C12N 2830/001 (2013.01); C12N 2830/85 (2013.01);
Abstract

A PDL1 block CAR-T transgenic vector for suppressing immune escape includes: AmpR sequence containing ampicillin resistance gene (SEQ ID NO: 1); prokaryotic replicon pUC Ori sequence (SEQ ID NO: 2); virus replicon SV40 Ori sequence (SEQ ID NO: 3); eWPRE enhanced posttranscriptional regulatory element of hepatitis B virus (SEQ ID NO: 11); human EF1a promoter (SEQ ID NO: 12); lentiviral packaging cis-elements for lentiviral packaging; humanized single-chain antibody fragment PDL1scFv1 (SEQ ID NO: 21), PDL1scFv2 (SEQ ID NO: 22), or PDL1scFv3 (SEQ ID NO: 23) of human PDL1; IRES ribosome binding sequence (SEQ ID NO: 25); IL6 signal peptide (SEQ ID NO: 26); human antibody Fc segment (SEQ ID NO: 27); and chimeric antigen receptors of the second or third generation CAR for integrating recognition, transmission and initiation. A preparation method of the PDL1 block CAR-T transgenic vector and an application thereof in a preparation of anti-immune escape drugs.

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