The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 04, 2020

Filed:

Jun. 27, 2012
Applicants:

Lennart Bjorkesten, Uppsala, SE;

Sofia Edlund, Uppsala, SE;

Asa Hagner-mcwhirter, Uppsala, SE;

Inventors:

Lennart Bjorkesten, Uppsala, SE;

Sofia Edlund, Uppsala, SE;

Asa Hagner-McWhirter, Uppsala, SE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/543 (2006.01); G01N 33/561 (2006.01); G01N 27/447 (2006.01);
U.S. Cl.
CPC ...
G01N 33/543 (2013.01); G01N 27/44726 (2013.01); G01N 33/561 (2013.01);
Abstract

The present invention relates to a method for identification of specific target proteins in a protein sample following a detection procedure, such as a Western blotting procedure, wherein the membrane is probed with at least two primary antibodies directed against the same and/or different epitopes of the same target protein, and wherein specific binding to the target protein in a sample is differentiated from unspecific binding to the target protein by comparing the resulting sample patterns, such as bands or spot patterns, with each other. In a further step signals from the true target proteins are enhanced while signals resulting from unspecific binding are diminished.


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