The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 28, 2020

Filed:

Mar. 28, 2014
Applicant:

The University of British Columbia, Vancouver, CA;

Inventors:

Marketa Ricicova, Vancouver, CA;

Kevin Albert Heyries, Vancouver, CA;

Hans Zahn, Munich, DE;

Oleh Petriv, Richmond, CA;

Veronique Lecault, Vancouver, CA;

Anupam Singhal, Mississauga, CA;

Daniel J. Da Costa, Vancouver, CA;

Carl L. G. Hansen, Vancouver, CA;

Brad Nelson, Victoria, CA;

Julie Nielsen, Victoria, CA;

Kathleen Lisaingo, Port Moody, CA;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01); B01L 3/00 (2006.01); C07K 16/40 (2006.01); C07K 16/10 (2006.01); C07K 16/00 (2006.01); C12N 5/078 (2010.01); C12N 5/0781 (2010.01); C12Q 1/6888 (2018.01); G01N 35/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5052 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502738 (2013.01); B01L 3/502761 (2013.01); C07K 16/00 (2013.01); C07K 16/1018 (2013.01); C07K 16/40 (2013.01); C12N 5/0634 (2013.01); C12N 5/0635 (2013.01); C12Q 1/6888 (2013.01); G01N 33/505 (2013.01); G01N 33/5047 (2013.01); G01N 33/54313 (2013.01); G01N 33/6845 (2013.01); G01N 33/6854 (2013.01); G01N 35/0099 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/12 (2013.01); B01L 2300/04 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0838 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/14 (2013.01); C07K 2317/14 (2013.01); C07K 2317/21 (2013.01); C07K 2317/70 (2013.01);
Abstract

Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.


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