The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 14, 2020

Filed:

Jan. 20, 2017
Applicant:

Temple University-of the Commonwealth System of Higher Education, Philadelphia, PA (US);

Inventors:

Nancy Pleshko, Cherry Hill, NJ (US);

Arash Hanifi, King of Prussia, PA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/00 (2006.01); G01N 21/00 (2006.01); G01N 27/447 (2006.01); C07K 1/26 (2006.01); G01N 21/359 (2014.01); G01N 21/64 (2006.01); G01N 21/65 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44721 (2013.01); C07K 1/26 (2013.01); G01N 21/359 (2013.01); G01N 21/6428 (2013.01); G01N 21/65 (2013.01); G01N 27/44782 (2013.01); G01N 2021/6439 (2013.01);
Abstract

The present invention introduces a protein/peptide quantification method based on near infrared spectral imaging of protein gel bands obtained from gel electrophoresis methodologies. Spectral imaging is a fast and reliable method that is simple to use and easily applicable to several procedures. When proteins are separated through gel electrophoresis, the gel is imaged using a near infrared spectrometer and a standard curve is used to calculate the protein/peptide content based on its relative near infrared absorbance to standard proteins. Compared to other protein quantification techniques, the methods of the present invention quantifies the proteins separated by gel electrophoresis without the need for contrast reagents or for purifying protein out of the gel, shortening the processing time, reducing the number of steps involved, and eliminating the need for additional chemicals.


Find Patent Forward Citations

Pleshko, Nancy. (2020). Protein quantification by near infrared spectral imaging (U.S. Patent No. 10712310). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/10712310

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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