The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 14, 2020

Filed:

Apr. 03, 2018
Applicants:

Guizhou Medical University, Guiyang, CN;

Maternal and Child Health Hospital of Guiyang City, Guiyang, CN;

Guizhou Entry-exit Inspection and Quarantine Bureau, Guiyang, CN;

Inventors:

Wenfeng Yu, Guiyang, CN;

Shi Zhou, Guiyang, CN;

Zhi Huang, Guiyang, CN;

Bi Wang, Guiyang, CN;

Rui Gao, Guiyang, CN;

Dai Wei Zhao, Guiyang, CN;

Hai Zhuang, Guiyang, CN;

Ya Ling Liang, Guiyang, CN;

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C07D 227/02 (2006.01); C12Q 1/6886 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6886 (2013.01); C07D 227/02 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6869 (2013.01);
Abstract

A kit for determining ctDNA concentration and a method for determining ctDNA concentration is disclosed, wherein the method comprising dissolving extracted ctDNA in Tris-HCl buffer with pH>7.0 and adding into the buffer 2,7-bis(1-methyl-4-vinylpyridine)-9-ethylcarbazole iodised salt as a molecular probe, calculating the concentration of ctDNA in the solution by measuring the Fluorescence intensity of the solution. It has extremely high sensitivity in the determination of 0 μg/ml˜50 μg/ml ctDNA solution.


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