The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 14, 2020

Filed:

Aug. 19, 2015
Applicant:

Fujifilm Wako Pure Chemical Corporation, Osaka-shi, Osaka, JP;

Inventor:

Yukinobu Hayashida, Amagasaki, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6881 (2018.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01); C12N 15/09 (2006.01); C07K 14/47 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6881 (2013.01); C07K 14/4702 (2013.01); C12N 15/09 (2013.01); G01N 33/50 (2013.01); G01N 33/5073 (2013.01); G01N 33/68 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01);
Abstract

The invention relates to a method which is capable of determining, at an initial stage of differentiation, the differentiated state of undifferentiated stem cells. The invention provides a method for determining a differentiated state of a cell comprising detecting expression of FOXB2 gene of a stem cell, and determining the differentiated state based on the result, and a differentiation marker selected from mRNA or protein derived from the FOXB2 gene. The invention is applicable to quality management of the stem cells or to methods for preparation and isolation of the differentiated cells. Further, because the differentiated cells can be determined at an initial stage of culture, the invention is useful for an early stage screening of cells and for quality management of the stem cells, and reduction in culturing period and cost reduction in expenditure regarding a culture medium, or the like, can be expected.


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