The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 14, 2020

Filed:

Oct. 06, 2009
Applicants:

Ingo Roehl, Memmelsdorf, DE;

Markus Schuster, Bayreuth, DE;

Stephan Seiffert, Kulmbach, CH;

Inventors:

Ingo Roehl, Memmelsdorf, DE;

Markus Schuster, Bayreuth, DE;

Stephan Seiffert, Kulmbach, CH;

Assignee:

AXOLABS GMBH, Kulmbach, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6816 (2018.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6816 (2013.01); C12Q 1/6816 (2013.01); C12Q 2525/107 (2013.01); C12Q 2525/207 (2013.01); C12Q 2563/107 (2013.01); C12Q 2565/137 (2013.01);
Abstract

The invention relates to a method for the detection of oligonucleotides using anion exchange high performance liquid chromatography. Fluorescently labelled peptide nucleic acid oligomers, complementary to the oligonucleotide are hybridized to the oligonucleotides. Anion exchange high performance liquid chromatography is then performed and the hybridized moieties detected and quantitated. The invention also relates to a method for the simultaneous detection of both strands of an oligonucleotide in parallel from one sample, and a kit for use in qualitative and quantitative detection of one or two strands of an oligonucleotide.


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