The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 14, 2020

Filed:

Jul. 22, 2019
Applicant:

Jiangnan University, Wuxi, CN;

Inventors:

Pu Zheng, Wuxi, CN;

Dan Wu, Wuxi, CN;

Peng Wen, Wuxi, CN;

Pengcheng Chen, Wuxi, CN;

Yin Fu, Wuxi, CN;

Assignee:

Jiangnan University, Wuxi, Jiangsu, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 17/04 (2006.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01);
U.S. Cl.
CPC ...
C12P 17/04 (2013.01); C12N 9/0006 (2013.01); C12N 9/0077 (2013.01); C12Y 101/99033 (2013.01);
Abstract

The present application discloses a method for producing piperonal by using a recombinant engineered bacterium co-expressing trans-anethole oxygenase and formate dehydrogenase, and an engineered bacterium thereof, including constructing a formate dehydrogenase gene fdh and trans-anethole oxygenase gene tao or trans-anethole oxygenase mutant gene co-expression recombinant vector; inductively expressing recombinant genetically engineered bacterium; and producing piperonal by using the recombinant genetically engineered bacterium. 15.91 g/L of piperonal with a transformation rate of 79.55% and a time-space transformation rate of 2.27 g/L/h can be finally obtained during catalysis, and the yield is significantly improved compared with the existing piperonal, thereby being more conducive to the smooth realization of industrial production.


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Zheng, Pu. (2020). Recombinant engineered bacterium co-expressing trans-anethole oxygenase and formate dehydrogenase and application thereof in production of piperonal (U.S. Patent No. 10711291). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/10711291

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