The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 14, 2020

Filed:

Jan. 25, 2017
Applicant:

Shanghai Jiao Tong University, Shanghai, CN;

Inventors:

Ping Xu, Shanghai, CN;

Chao Li, Shanghai, CN;

Fei Tao, Shanghai, CN;

Hongzhi Tang, Shanghai, CN;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 7/56 (2006.01); C12N 15/75 (2006.01); C12N 9/10 (2006.01); C12N 9/04 (2006.01); C12N 9/88 (2006.01);
U.S. Cl.
CPC ...
C12P 7/56 (2013.01); C12N 9/0006 (2013.01); C12N 9/1022 (2013.01); C12N 15/75 (2013.01); C12Y 101/01028 (2013.01);
Abstract

Provided herein is D-lactate dehydrogenase, an engineered strain containing the D-lactate dehydrogenase, and a construction method and use of the engineered strain. The D-lactate dehydrogenase has unique properties and is from, and the D-lactate dehydrogenase has good thermophily and heat stability. By using the D-lactate dehydrogenase and the gene engineering reconstruction method, a fermentation product of the reconstructedcan be redirected to optically-pure D-lactic acid with a high yield from naturally produced 2,3-butanediol, and the optical purity of the produced D-lactic acid reaches 99.9%; and raw materials for fermentation are low-cost, and a fermentation state is between an anaerobic fermentation state and a microaerobic fermentation state. By using the method for producing D-lactic acid through fermentation at high temperature, the production cost can be reduced, the production efficiency can be improved and there is a wide industrial application prospect for the method.


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