The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 2020
Filed:
Jun. 08, 2016
Applicant:
National Institute of Advanced Industrial Science and Technology, Tokyo, JP;
Inventors:
Yoshio Kato, Tsukuba, JP;
Naoshi Kojima, Tsukuba, JP;
Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/113 (2010.01); C12N 15/09 (2006.01); C12N 15/67 (2006.01); C12N 9/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/113 (2013.01); C12N 9/00 (2013.01); C12N 15/09 (2013.01); C12N 15/67 (2013.01); C12N 2310/111 (2013.01); C12N 2310/12 (2013.01);
Abstract
Provided is a method for synthesizing a protein, into which a nucleobase amino acid (NBA) is introduced at a desired position, that comprises: a step for preparing mRNA into which a modified codon is inserted at a desired position downstream of an initiation codon; and a step for translating the aforesaid mRNA into a protein in the presence of tRNA, said tRNA recognizing the modified codon and being acylated with the NBA. Also provided is a ribozyme that catalyzes the aminoacylation of tRNA and comprises two RNA molecules.