The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 2020
Filed:
May. 12, 2014
The University of Tokyo, Tokyo, JP;
Peptidream Inc., Kanagawa, JP;
Hiroshi Murakami, Tokyo, JP;
Takashi Kawakami, Tokyo, JP;
Patrick Reid, Tokyo, JP;
Toru Sasaki, Tokyo, JP;
The University of Tokyo, Tokyo, JP;
PeptiDream Inc., Kanagawa, JP;
Abstract
An object of the present invention is to provide a method for producing a peptide library capable of incorporating an arbitrary number of arbitrary proteinogenic and/or non-proteinogenic amino acids in an arbitrary site. The invention provides a method for producing a peptide library including 1×10or more kinds of peptides containing amino acids encoded by NNN, including a step of preparing an mRNA library including mRNAs which encode peptides of the peptide library and each contain at least one NNN; and a step of translating each mRNA of the mRNA library in a cell-free translation system added with tRNA containing an anticodon to any one of NNNcodons and charged with an amino acid corresponding to the codon (wherein, N, N, and Nare each independently selected from adenine (A), guanine (G), cytosine (C), and uracil (U) and an arbitrary amino acid is reassigned to each NNN).