The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 14, 2020

Filed:

Jan. 11, 2018
Applicant:

National Chi Nan University, Nantou County, TW;

Inventors:

Yung-Pin Tsai, Nantou County, TW;

Meng-Shan Lu, Kaohsiung, TW;

Chih-Chi Yang, Miaoli County, TW;

Hao Shiu, New Taipei, TW;

Jan-Wei Lin, Nantou County, TW;

Assignee:

NATIONAL CHI NAN UNIVERSITY, Nantou County, TW;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 13/00 (2006.01); C08G 63/89 (2006.01); C12N 1/06 (2006.01); C02F 11/00 (2006.01); C02F 1/26 (2006.01); C02F 103/00 (2006.01); C02F 103/30 (2006.01); C02F 103/20 (2006.01); C02F 11/12 (2019.01);
U.S. Cl.
CPC ...
C12N 13/00 (2013.01); C02F 1/26 (2013.01); C02F 11/006 (2013.01); C08G 63/89 (2013.01); C12N 1/06 (2013.01); C02F 11/12 (2013.01); C02F 2103/003 (2013.01); C02F 2103/20 (2013.01); C02F 2103/30 (2013.01); C02F 2201/46135 (2013.01); C02F 2201/46175 (2013.01); C02F 2209/02 (2013.01); C02F 2209/06 (2013.01); C02F 2209/44 (2013.01);
Abstract

The present invention provides a method for extracting polyhydroxyalkanoates (PHAs), which comprises a pre-process step and an extraction step: removing water from waste sludge containing microorganisms in the pre-process step so that the waste sludge containing microorganisms has a water content of less than 40%; and applying a high-voltage pulsed electric field to the waste sludge during the extraction step to destroy the microorganisms and release the PHAs, wherein the high-voltage pulsed electric field is between 50 volts and 400 volts, an application time of the high-voltage pulsed electric field is between 5 seconds and 90 seconds, and an application frequency of the high-voltage pulsed electric field is between 500 Hz and 1000 Hz, thereby extracting the PHAs in the case of few chemicals.


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