The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 2020
Filed:
Dec. 25, 2015
Applicant:
Kyoto University, Kyoto-shi, Kyoto, JP;
Inventors:
Kenji Osafune, Kyoto, JP;
Maki Kotaka, Kyoto, JP;
Assignee:
KYOTO UNIVERSITY, Kyoto, JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/067 (2013.01); G01N 33/5067 (2013.01); C12N 2500/30 (2013.01); C12N 2500/62 (2013.01); C12N 2501/12 (2013.01); C12N 2501/16 (2013.01); C12N 2501/237 (2013.01); C12N 2501/31 (2013.01); C12N 2501/39 (2013.01); C12N 2501/727 (2013.01); C12N 2501/73 (2013.01); C12N 2501/80 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01);
Abstract
A method for producing hepatocytes from hepatoblasts is provided. The method includes the step of culturing the hepatoblasts in a medium containing a compound selected from the group consisting of pregnenolone and an adrenergic agonist. The hepatoblasts can be obtained by culturing endodermal cells in a medium containing DMSO, and the endodermal cells can be obtained by culturing pluripotent stem cells in a medium containing Activin A and a GSK-3β inhibitor. Accordingly, a method for producing hepatocytes from pluripotent stem cells is also provided by employing the method of the present invention.