The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 2020
Filed:
Dec. 17, 2014
Cytoo, Grenoble, FR;
Mathieu Fernandes, Saint-Jean de Moirans, FR;
Sebastien Degot, Grenoble, FR;
Yoran Margaron, Voiron, FR;
Jie Liu, Grenoble, FR;
Michel Bornens, Sceaux, FR;
Maria Luisa Calvo Munoz, Grenoble, FR;
Aurelie Berthelot, Echirolles, FR;
Alexandra Fuchs, Beaulieu, FR;
Joanne Young, Voreppe, FR;
Delphine Morales, Grenoble, FR;
CYTOO, Grenoble, FR;
Abstract
A method and device for standardizing myoblast differentiation into myotubes, including a substrate () and at least one cell-adhesive pattern () for culturing myoblasts on the substrate. The pattern () has an elongated surface. A central region (C) and two lateral regions (L) extend from the central region in both directions along a longitudinal axis of the pattern. The ratio between the maximum width (W) of the central region (C) and the maximum width (W) of the lateral regions (L) is greater than or equal to 2. The ratio between the length (L) and the maximum width (W) of the pattern () is less than or equal to 4. The method includes providing a device as described above, depositing myoblasts on at least one cell-adhesive pattern of the device, culturing the myoblasts in a differentiation medium to promote cell differentiation into myotubes and constrain elongation of the myotubes.