The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 07, 2020

Filed:

Mar. 17, 2014
Applicant:

Iris International, Inc., Chatsworth, CA (US);

Inventors:

Bart J. Wanders, Trabuco Canyon, CA (US);

Brett Jordan, Los Angeles, CA (US);

Gregory A. Farrell, Ridgewood, NJ (US);

Thomas H. Adams, Encinitas, CA (US);

Warren Groner, Great Neck, NY (US);

Assignee:

Iris International, Inc., Chatsworth, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 15/14 (2006.01); G01N 21/00 (2006.01); G01N 1/30 (2006.01); G01N 15/06 (2006.01); G01N 21/53 (2006.01); G01N 33/49 (2006.01); G01N 33/50 (2006.01); G01N 33/80 (2006.01); G01N 21/05 (2006.01); G01N 15/00 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
G01N 15/1404 (2013.01); G01N 1/30 (2013.01); G01N 15/06 (2013.01); G01N 15/147 (2013.01); G01N 15/1434 (2013.01); G01N 15/1463 (2013.01); G01N 15/1468 (2013.01); G01N 15/1475 (2013.01); G01N 21/53 (2013.01); G01N 33/49 (2013.01); G01N 33/4915 (2013.01); G01N 33/5091 (2013.01); G01N 33/5094 (2013.01); G01N 33/80 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/1037 (2013.01); G01N 2015/1411 (2013.01); G01N 2015/1413 (2013.01); G01N 2015/1452 (2013.01); G01N 2015/1486 (2013.01); G01N 2021/058 (2013.01);
Abstract

Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. A contrast pattern for autofocusing is provided on the flowcell, for example at an edge of a rear illumination opening. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Blood cell images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.


Find Patent Forward Citations

Loading…