The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 07, 2020

Filed:

Feb. 26, 2016
Applicant:

Aj Innuscreen Gmbh, Berlin, DE;

Inventors:

Timo Hillebrand, Hoppegarten, DE;

Thorsten Stroh, Berlin, DE;

Assignee:

AJ Innuscreen GmbH, Berlin, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/02 (2006.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); G01N 1/40 (2006.01); G01N 35/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1006 (2013.01); B01L 3/0275 (2013.01); C12N 15/1013 (2013.01); C12Q 1/6806 (2013.01); G01N 1/405 (2013.01); G01N 35/10 (2013.01); B01L 2200/023 (2013.01); B01L 2200/0631 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0854 (2013.01); B01L 2300/16 (2013.01);
Abstract

Device and method for extraction of nucleic acids, comprising a hollow body, preferably a pipette tip, through which a liquid is passed, characterized in that a material with rough or structured surface is disposed in this hollow body in such a way that it can be circumcirculated by a liquid. After lysis of the sample and adjustment of necessary binding conditions for adsorption of the nucleic acids on the carrier material, the mixture, by means of pipetting processes, is repeatedly 'pipetted along' the material for binding nucleic acids, introduced vertically in the pipette tip. The nucleic acids bind to the material. Thereupon washing buffers are likewise 'pipetted along' the material for binding nucleic acids. Then a drying step is performed. Finally, the eluent is again repeatedly 'pipetted along' the vertically disposed material for binding nucleic acids, and in the process the bound nucleic acid is detached. The nucleic acid is now available for necessary downstream application.


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