The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 07, 2020

Filed:

Dec. 27, 2017
Applicant:

Tokai Bussan Co., Ltd., Chiyoda-ku, Tokyo, JP;

Inventor:

Kuniki Kino, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/00 (2006.01); C12N 15/00 (2006.01); A61K 38/03 (2006.01); C12P 21/00 (2006.01); C12P 21/02 (2006.01); C07K 5/078 (2006.01); C07K 5/02 (2006.01);
U.S. Cl.
CPC ...
C12N 9/93 (2013.01); C07K 5/06147 (2013.01); C12P 21/02 (2013.01); C12Y 603/02028 (2013.01); C07K 5/0202 (2013.01);
Abstract

The present invention provides L-amino acid α-ligase represented by sequence number 1 (SEQ ID NO. 1), which is a mutant protein including substitution of at least 1-3 amino acid residues of amino acid sequence of protein YwfE, with the substitution being at least one of an asparagine (N) residue of the 108th place from the N terminal being substituted with at least one of an alanine (A) residue, a glutamic acid (E) residue and a glutamine (Q) residue, an isoleucine (I) residue of the 112th place from the N terminal being substituted with a valine (V) residue, and a histidine (H) residue of the 378th place from the N terminal being substituted with at least one of a lysine (K) residue or an arginine (R) residue.


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