The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 07, 2020
Filed:
Aug. 10, 2015
Applicant:
The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);
Inventors:
Calvin Jay Kuo, Palo Alto, CA (US);
Akifumi Ootani, Stanford, CA (US);
Assignee:
The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12N 5/00 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0679 (2013.01); C12N 5/0012 (2013.01); G01N 33/5005 (2013.01); G01N 33/5073 (2013.01); C12N 2501/415 (2013.01); C12N 2533/54 (2013.01);
Abstract
Methods are provided for long term culture of mammalian intestinal cells. Cultures are initiated with fragments of mammalian intestinal tissue, which are then maintained embedded in a gel substrate that provides an air-liquid interface. Intestinal epithelium in cultures of the invention can be continuously grown for extended periods of time. Mammalian intestinal cells cultured by the methods of the invention recapitulate features of intestinal growth in vivo.