Detection method for quaternary ammonium compound having γ-carboxyl group

Abstract

A detection method for a quaternary ammonium compound having a γ-carboxyl group includes: mixing a sample including the quaternary ammonium compound having the γ-carboxyl group, a fluorescent compound having a fluorophore and a polar aprotic solvent to form a reaction solution. A S2 nucleophilic substitution reaction is performed between the fluorophore of the fluorescent compound and the γ-carboxyl group of the quaternary ammonium compound to obtain a derivation solution including a derivative which is a quaternary ammonium compound with the γ-carboxyl group substituted with the fluorophore of the fluorescent compound. An extractant and a cosolvent are added to the derivation solution to form a water-in-oil emulsion which disperses in the derivation solution, followed by extracting the derivative from the derivation solution by the extractant to obtain an analytic solution, wherein a polarity of the extractant is higher than a polarity of the polar aprotic solvent. The derivative in the analytic solution is detected; and therefore, the quaternary ammonium compound having the γ-carboxyl group in the sample can be easily detected by reverse-phase liquid chromatography, fluorescence spectrometry or mass spectrometry.