The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 30, 2020
Filed:
Nov. 24, 2015
Applicant:
Quest Diagnostics Investments Incorporated, Wilmington, DE (US);
Inventors:
Lucien Jacky, Orange, CA (US);
Albert Castro, Walnut, CA (US);
Peter Lee, Tustin, CA (US);
Jose Matud, Long Beach, CA (US);
Michelle Tabb, Santa Ana, CA (US);
Assignee:
Quest Diagnostics Investments Incorporated, Wilmington, DE (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6806 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/6853 (2018.01); C12Q 1/686 (2018.01); C12N 1/06 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12N 1/06 (2013.01); C12N 15/1003 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/6853 (2013.01); C12Q 2523/32 (2013.01); C12Q 2565/629 (2013.01); Y02A 50/53 (2018.01);
Abstract
Disclosed are methods for identifying the presence or absence of a target nucleic acid from one or more organisms in a biological sample, said method comprising: (a) spinning the sample at a rotational velocity sufficient to pellet cellular debris and fluorescence inhibitors present within the sample and reduce fluorescence interference or quenching in the sample; and (b) directly amplifying and detecting the target nucleic acid in the sample.