The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 30, 2020
Filed:
Sep. 28, 2018
Applicant:
Bioo Scientific Corporation, Austin, TX (US);
Inventor:
Kevin Allen, Austin, TX (US);
Assignee:
BIOO SCIENTIFIC CORPORATION, Austin, TX (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6855 (2018.01); C12P 19/34 (2006.01); C12Q 1/686 (2018.01); C12N 9/12 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12N 9/127 (2013.01); C12N 9/1276 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6869 (2013.01);
Abstract
This disclosure provides, among other things, a method for making a cDNA library. In some embodiments the method may comprise adding a polyA tail to the longer RNA fragments but not the shorter RNA fragments in a sample by incubating the population of RNA fragments with a polyA polymerase, wherein the reaction conditions used preferentially tail only the longer fragments but not the shorter fragments.