The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 30, 2020

Filed:

Apr. 29, 2016
Applicant:

Bayer Aktiengesellschaft, Leverkusen, DE;

Inventors:

Benjamin Maiser, Leverkusen, DE;

Peter Schwan, Leverkusen, DE;

Martin Lobedann, Köln, DE;

Volker Möhrle, Köln, DE;

Assignee:

BAYER AKTIENGESELLSCHAFT, Leverkusen, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 15/18 (2006.01); B01D 19/00 (2006.01); B01D 61/24 (2006.01); B01D 61/58 (2006.01); C07K 1/36 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); B01D 61/14 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
C12M 47/12 (2013.01); B01D 15/1864 (2013.01); B01D 19/0031 (2013.01); B01D 61/142 (2013.01); B01D 61/145 (2013.01); B01D 61/243 (2013.01); B01D 61/58 (2013.01); C07K 1/36 (2013.01); C07K 16/00 (2013.01); C12M 37/00 (2013.01); C12M 47/16 (2013.01); B01D 2311/12 (2013.01); B01D 2311/2626 (2013.01); B01D 2311/2649 (2013.01); B01D 2315/16 (2013.01);
Abstract

The invention provides a method for the continuous, microbe-reduced production and/or processing of a biopharmaceutical, biological macromolecular product from a heterogeneous cell culture-fluid mixture, comprising the steps of: (a) providing a particle-free fluid from a heterogeneous cell culture-fluid mixture containing the product, in the form of a product stream, (b) at least one filtration, providing a filtrate, (c) at least two chromatography steps for purifying the product, (d) at least one virus depletion, (e) at least one ultrafiltration and/or at least one diafiltration of the product stream of steps (b), (c) and/or (d), characterized in that the at least two chromatography steps from (c) comprise a purification via at least two chromatography columns and/or membrane adsorbers in each case and that the process is carried out in a closed and modular manner The invention further provides a corresponding modular system for carrying out said method.


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