The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 30, 2020

Filed:

Apr. 23, 2018
Applicant:

Tagcyx Biotechnologies Inc., Tokyo, JP;

Inventors:

Ichiro Hirao, Komae, JP;

Michiko Hirao, Yokohama, JP;

Shuang Liu, Yokohama, JP;

Iwao Nozawa, Yokohama, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/115 (2010.01); A61K 31/7088 (2006.01); C12N 15/11 (2006.01); C12N 15/67 (2006.01);
U.S. Cl.
CPC ...
A61K 31/7088 (2013.01); C12N 15/111 (2013.01); C12N 15/115 (2013.01); C12N 15/67 (2013.01); C12N 2310/13 (2013.01); C12N 2310/14 (2013.01); C12N 2310/3519 (2013.01); C12N 2320/51 (2013.01);
Abstract

This invention is intended to enhance and improve the resistance of a single- or double-stranded nucleic acid fragment comprising a base sequence of a functional nucleic acid to degradation by nucleolytic enzymes in a simple and cost-effective manner. The single- or double-stranded nucleic acid fragment comprises, ligated to at least one end thereof, hairpin-shaped DNA comprising: (A) a nucleic acid region comprising 2 to 5 arbitrary nucleotides; (B) a nucleic acid region comprising a 'gna' or 'gnna' base sequence, wherein each 'n' represents “g”, “t”, “a”, or “c”, a base analogue, or a modified base; and (C) a nucleic acid region comprising a base sequence complementary to the nucleic acid region (A), sequentially from the 5' end toward the 3′ end.


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