The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 23, 2020
Filed:
Nov. 17, 2016
Paris Sciences ET Lettres - Quartier Latin, Paris, FR;
Universite Paris Diderot Paris 7, Paris, FR;
Commissariat a L'energie Atomique ET Aux Energies Alternatives, Paris, FR;
Abdou Rachid Thiam, Choisy-le-roi, FR;
Béatrice Schaack, Grenoble, FR;
Abstract
A method to analyze the activity of an ion channel comprising: providing in a hydrophobic medium a first droplet of an aqueous solution AScomprising a concentration q of an ion I with c0, wherein the droplet is surrounded by a monolayer of amphiphilic molecules; providing in the hydrophobic medium a second droplet of an aqueous solution AScomprising a concentration cof the ion I with c0, and c≠c, wherein the second droplet is surrounded by a monolayer of amphiphilic molecules further comprising the ion channels to an analyzed; bringing the first droplet and the second droplet into contact so as to form a bilayer of amphiphilic molecules, wherein the bilayer further comprises ion channels to be analyzed, and measuring the radius of the two droplets when they are brought into contact referred to as initial state; and maintaining the first droplet and the second droplet in contact until equilibrium is reached and measuring the radius of the two contacted droplets or determining the number of resulting droplet(s) at the equilibrium state, wherein the ion channel is inactive when the difference of the radius of at least one droplet between its initial state and its equilibrium state is at least 10% or when only one droplet is obtained at the equilibrium state.