The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 16, 2020

Filed:

Dec. 01, 2016
Applicant:

Panasonic Intellectual Property Management Co., Ltd., Osaka, JP;

Inventors:

Yasuaki Okumura, Kyoto, JP;

Tatsurou Kawamura, Kyoto, JP;

Noriko Shimba, Nara, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 27/447 (2006.01); B03C 5/00 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44726 (2013.01); B03C 5/005 (2013.01); G01N 33/5438 (2013.01);
Abstract

The present invention provides a method for detecting an analyte with high sensitivity. In the present method, a solution is supplied onto a substrate comprising a first electrode and a second electrode. Then, an alternating voltage is applied between the first electrode and the second electrode to aggregate, onto the surface between the first electrode and the second electrode by dielectrophoresis, bioparticles and dielectric particles contained in the solution. The aggregated bioparticles are broken to release the analyte contained in the bioparticles. The released analyte is bound to a first antibody and a second antibody to cause the dielectric particles to be immobilized onto the substrate through formation of a sandwich structure composed of the first antibody, the analyte, and the second antibody. Finally, the analyte is detected through the fluorescent substance contained in the immobilized dielectric particles.


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