The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 16, 2020

Filed:

Jun. 04, 2014
Applicant:

Autodisplay Biotech Gmbh, Duesseldorf, DE;

Inventors:

Joachim Jose, Duesseldorf, DE;

Mark George Teese, Muenster, DE;

Shanna Sichwart, Muenster, DE;

Iasson Tozakidis, Muenster, DE;

Assignee:

Autodisplay Biotech GmbH, Duesseldorf, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/62 (2006.01); C12N 9/42 (2006.01); C12N 15/10 (2006.01); C12N 15/63 (2006.01);
U.S. Cl.
CPC ...
C12N 15/625 (2013.01); C12N 9/2437 (2013.01); C12N 9/2445 (2013.01); C12N 15/1037 (2013.01); C12N 15/63 (2013.01); C07K 2319/02 (2013.01); C07K 2319/035 (2013.01); C07K 2319/60 (2013.01); C12Y 302/01004 (2013.01); C12Y 302/01021 (2013.01); C12Y 302/01091 (2013.01);
Abstract

The present invention relates to a method for the surface display of a recombinant polypeptide on the surface of a host cell, said method comprising the steps: (a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide, (ii) a portion encoding the recombinant polypeptide to be displayed, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the trans porter domain of an EhaA protein, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell.


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