The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 16, 2020

Filed:

Feb. 20, 2018
Applicants:

Yi Xiao, Miami, FL (US);

Haixiang Yu, Miami, FL (US);

Inventors:

Yi Xiao, Miami, FL (US);

Haixiang Yu, Miami, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 15/115 (2010.01); C09B 23/01 (2006.01); C07C 225/10 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 15/115 (2013.01); C07C 225/10 (2013.01); C09B 23/0066 (2013.01); G01N 33/50 (2013.01); C12N 2310/16 (2013.01); C12N 2310/3517 (2013.01); C12N 2320/13 (2013.01);
Abstract

The subject invention provides methods, assays, and products for detecting small molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target, preferably, a synthetic cathinone in a sample comprises contacting the sample with an aptamer-based sensor selective for the small-molecule target, and detecting the small-molecule target in the sample. Specifically, the method utilizes an aptamer-based sensor comprising a dye binding to a three-way junction binding domain of an aptamer. Binding of small-molecule target to the aptamer displaces the dye, generating a spectroscopic signal that can be used for detection of the small-molecule target and quantitative measurement of the target concentration.


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