The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 09, 2020

Filed:

Sep. 27, 2013
Applicant:

Corning Incorporated, Corning, NY (US);

Inventors:

Kaoru Kobayashi, Chiba, JP;

Hanaka Mimura, Chiba, JP;

Kan Chiba, Chiba, JP;

Yoko Ejiri, Kurashiki, JP;

Masaya Hosoda, Kurashiki, JP;

Satoru Ayano, Kurashiki, JP;

Assignee:

Corning Incorporated, Corning, NY (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/26 (2006.01); C12Q 1/6876 (2018.01); G01N 33/50 (2006.01); G01N 33/573 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5041 (2013.01); C12Q 1/26 (2013.01); C12Q 1/6876 (2013.01); G01N 33/5073 (2013.01); G01N 33/573 (2013.01); C12Q 2600/136 (2013.01); C12Q 2600/158 (2013.01); G01N 2333/485 (2013.01); G01N 2333/525 (2013.01); G01N 2333/5412 (2013.01); G01N 2333/80 (2013.01); G01N 2500/04 (2013.01); G01N 2500/10 (2013.01);
Abstract

Provided is a technique that uses an established hepatocyte cell line in a method for evaluating an effect of a cytokine on a metabolic activity of a cytochrome P450 and in a method for evaluating a drug which interacts with a cytokine. The method for evaluating an effect of a cytokine on a metabolic activity of a cytochrome P450 includes: culturing an established hepatocyte cell line by using a culture chamber () including culture rooms (), to thereby form spheroids (); and evaluating the presence or absence of induction or attenuation of the cytochrome P450 after bringing a spheroid-shaped established hepatocyte cell line into contact with a test solution containing the cytokine in the culture chamber for one hour or more and less than 96 hours.


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