The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 02, 2020
Filed:
May. 31, 2017
Applicant:
Sangamo Therapeutics, Inc., Richmond, CA (US);
Inventors:
Dmitry Guschin, Richmond, CA (US);
Fyodor Urnov, Richmond, CA (US);
Assignee:
Sangamo Therapeutics, Inc., Richmond, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C07K 14/47 (2006.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C07K 14/4702 (2013.01); C12N 9/22 (2013.01); C12N 15/10 (2013.01); C07K 2319/81 (2013.01);
Abstract
Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a cell such that two targeted cleavage events occur. Subsequent cellular repair mechanisms result in deletion of sequences between the two cleavage sites.