The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 02, 2020

Filed:

Jun. 24, 2016
Applicants:

Children's Medical Center Corporation, Boston, MA (US);

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Derrick J. Rossi, Newton, MA (US);

Wataru Ebina, Boston, MA (US);

Morag Stewart, Boston, MA (US);

Paula Gutierrez-Martinez, Brookline, MA (US);

Lee L. Rubin, Wellesley, MA (US);

Lance Davidow, Lexington, MA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0789 (2010.01); A61K 35/28 (2015.01); A61K 38/19 (2006.01); C12N 15/86 (2006.01); C07K 14/495 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0647 (2013.01); A61K 35/28 (2013.01); A61K 38/193 (2013.01); C12N 15/86 (2013.01); C07K 14/495 (2013.01); C12N 2500/02 (2013.01); C12N 2500/38 (2013.01); C12N 2500/62 (2013.01); C12N 2500/99 (2013.01); C12N 2501/065 (2013.01); C12N 2501/115 (2013.01); C12N 2501/125 (2013.01); C12N 2501/145 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/2303 (2013.01); C12N 2501/2312 (2013.01); C12N 2501/26 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2510/02 (2013.01);
Abstract

The methods and compositions described herein relate to producing, expanding, enriching, and/or maintaining hematopoietic stem cells ex vivo by treating the cells with an agent(s) that exhibits two or more activities selected from modulation of histone methylation; inhibition of TGFβ signaling; inhibition of p38 signaling; activation of canonical Wnt signaling; and modulation of histone acetylation. In some embodiments, the technology described herein relates to transplantation of hematopoietic stem cells.


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