The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 26, 2020
Filed:
May. 26, 2017
Ventana Medical Systems, Inc., Tucson, AZ (US);
Oregon Health & Science University, Portland, OR (US);
Michael Barnes, Oro Valley, AZ (US);
David Chafin, Tucson, AZ (US);
Karl Garsha, Sahuarita, AZ (US);
Thomas M. Grogan, Tucson, AZ (US);
Esteban Roberts, Tucson, AZ (US);
Benjamin Stevens, Oro Valley, AZ (US);
Franklin Ventura, Tucson, AZ (US);
Christophe Chefd'hotel, Sunnyvale, CA (US);
Kandavel Shanmugam, Chandler, AZ (US);
Joe Gray, Lake Oswego, AZ (US);
Damien Ramunno-Johnson, Vancouver, WA (US);
Tania Vu, Portland, OR (US);
Brian J. Druker, Portland, OR (US);
Thomas Jacob, Beaverton, OR (US);
VENTANA MEDICAL SYSTEMS, INC., Tucson, AZ (US);
OREGON HELATH & SCIENCE UNIVERSITY, Portland, OR (US);
Abstract
Methods, systems, and apparatuses for detecting and describing heterogeneity in a cell sample are disclosed herein. A plurality of fields of view (FOV) are generated for one or more areas of interest (AOI) within an image of the cell sample are generated. Hyperspectral or multispectral data from each FOV is organized into an image stack containing one or more z-layers, with each z-layer containing intensity data for a single marker at each pixel in the FOV. A cluster analysis is applied to the image stacks, wherein the clustering algorithm groups pixels having a similar ratio of detectable marker intensity across layers of the z-axis, thereby generating a plurality of clusters having similar expression patterns.