The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 26, 2020
Filed:
Oct. 31, 2017
Personal Genomics, Inc., Grand Cayman, KY;
Shiuan-Woei Lin Wu, New Taipei, TW;
Ching-Wei Tsai, Taoyuan, TW;
Ting-Yueh Tsai, Hsinchu, TW;
Jyun-Yuan Huang, Kaohsiung, TW;
Chao-Chi Pan, Hsinchu, TW;
Li-Chi Chang, Taipei, TW;
Ching-Long Hwong, Kaohsiung, TW;
PERSONAL GENOMICS, INC., Grand Cayman, KY;
Abstract
A composition comprising a recombinant DNA polymerase and 3'-esterified nucleotide analogues is provided. The recombinant DNA polymerase includes an amino acid sequence that is at least 90% homology with 9° N DNA polymerase (SEQ ID NO: 1), and the recombinant DNA polymerase includes at least two mutations at the positions corresponding to amino acid residues 141 and 143 of the 9° N DNA polymerase. A recombinant DNA polymerase is further provided. The recombinant DNA polymerase includes an amino acid sequence that is at least 90% homology with 9° N-III DNA polymerase (SEQ ID NO: 3), and the recombinant DNA polymerase includes one or two mutations at the positions corresponding to amino acid residue 480 and 486 of the 9° N-III DNA polymerase. A method of performing a polymerization reaction is also provided.