The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 26, 2020
Filed:
Sep. 09, 2016
Applicant:
Trustees of Tufts College, Medford, MA (US);
Inventors:
Dana Cairns, Somerville, MA (US);
David L. Kaplan, Concord, MA (US);
Assignee:
Trustees of Tufts College, Medford, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/0797 (2010.01); C12N 15/67 (2006.01); C12N 15/85 (2006.01); C12N 15/63 (2006.01); C12N 5/10 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0623 (2013.01); C12N 5/10 (2013.01); C12N 15/63 (2013.01); C12N 15/67 (2013.01); C12N 15/85 (2013.01); C12N 2740/15041 (2013.01);
Abstract
In some embodiments, the present invention provides methods including the steps of providing one or more human somatic cells, causing transient increased expression of OCT4, KLF4, SOX2, and cMYC in the somatic cells forming modified somatic cells, providing a plurality of inactivated embryonic fibroblasts, associating the modified somatic cells with the inactivated embryonic fibroblasts in a culture media comprising 20% KO DMEM xeno-free serum replacement and at least 15 ng/ml recombinant bFGF to form human induced neural stem cells.