The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 19, 2020

Filed:

Jun. 02, 2017
Applicant:

Konica Minolta, Inc., Chiyoda-ku, Tokyo, JP;

Inventors:

Yuichi Ozaki, Hino, JP;

Kensaku Takanashi, Hino, JP;

Daisuke Tomioka, Nishitokyo, JP;

Assignee:

Konica Minolta, Inc., Chiyoda-ku, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); G01N 33/58 (2006.01); G06T 1/00 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6458 (2013.01); G01N 21/64 (2013.01); G01N 33/54313 (2013.01); G01N 33/54346 (2013.01); G01N 33/582 (2013.01); G01N 33/585 (2013.01); G01N 33/587 (2013.01); G06T 1/00 (2013.01); G06T 1/0007 (2013.01);
Abstract

A method of quantifying a specific biological material in a specimen stained using fluorescent dye accumulating particles capable of binding to the material includes: inputting a first fluorescence image obtained by capturing an image of the specimen; extracting a certain region from the first fluorescence image and calculating a first luminance integrated value by integrating luminance values of the certain region; and calculating the number of the particles included in the certain region from the first luminance integrated value and an average luminance value per fluorescent dye accumulating particle, wherein the average luminance value is calculated from a distribution of second luminance integrated values obtained by integrating luminance values for individual bright spot regions, which indicate emission of light by the fluorescent dye accumulating particles, in a second fluorescence image obtained by capturing an image of a preparation onto which the fluorescent dye accumulating particles are dispersed without agglomerating.


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