The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 19, 2020
Filed:
Jul. 09, 2018
Applicant:
Beijing Jinhong Tianbang Information Technology Co., Ltd, Beijing, CN;
Inventors:
Yu Wang, Tianjin, CN;
Litong Ban, Tianjin, CN;
Liang Huang, Tianjin, CN;
Ning Sun, Tianjin, CN;
Hongpeng Yang, Tianjin, CN;
Assignee:
BEIJING HONGMING XINDA TECHNOLOGY CO., LTD., Beijing, CN;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/44 (2006.01); C12N 9/24 (2006.01); C12N 15/74 (2006.01); C12N 15/52 (2006.01); C12R 1/84 (2006.01); C12P 19/02 (2006.01); C12P 19/14 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2457 (2013.01); C12N 15/52 (2013.01); C12N 15/74 (2013.01); C12P 19/02 (2013.01); C12P 19/14 (2013.01); C12R 1/84 (2013.01); C12Y 302/01041 (2013.01);
Abstract
The present application relates to the field of enzyme engineering, especially relates to a pullulanase as well as preparation and use thereof. The pullulanase and coding gene thereof were obtained by random mutation by using the Error-prone PCR technique on the gene of wild-type pullulanase to obtain a mutant PLUM. The enzyme activity of the mutant PLUM was improved by 57.03% compared with the wild-type pullulanase PLUM.