The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 12, 2020

Filed:

Feb. 05, 2016
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Thomas Wessel, Pleasanton, CA (US);

Yong Chu, Castro Valley, CA (US);

Jacob Freudenthal, San Jose, CA (US);

David Woo, Foster City, CA (US);

Assignee:

Life Technologies Corporation, Carlsbad, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); G01N 21/27 (2006.01); G16B 40/00 (2019.01);
U.S. Cl.
CPC ...
G01N 21/6428 (2013.01); G01N 21/274 (2013.01); G01N 21/6452 (2013.01); G01N 21/6456 (2013.01); G01N 2021/6439 (2013.01); G16B 40/00 (2019.02);
Abstract

In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (C) values of amplification curves generated from the samples of the first quantity and a second set of Cvalues of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Cvalues at each of the plurality of fluorescence thresholds.


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