The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 05, 2020
Filed:
Feb. 20, 2016
Takara Bio Usa, Inc., Mountain View, CA (US);
Alain-Albert Mir, Redwood City, CA (US);
Thomas D. Schaal, San Francisco, CA (US);
Chun-Wah Lin, Fremont, CA (US);
Shanavaz Loharasp D. Nasarabadi, Fremont, CA (US);
Jude Dunne, Menlo Park, CA (US);
Maithreyan Srinivasan, Palo Alto, CA (US);
Patricio Espinoza, Fremont, CA (US);
TAKARA BIO USA, INC., Mountain View, CA (US);
Abstract
The present disclosure provides methods, device, assemblies, and systems for dispensing and visualizing single cells. For example, provided herein are systems and methods for dispensing a dispense volume into a plurality of wells of a multi-well device, where, on average, a pre-determined number of cells (e.g., 1-20) are present in the dispense volume, and determining, via a cellular label, the number of cells present in each of the plurality of wells. Such dispensing and cell detection may be repeated a number of times with respect to wells identified as having less than the pre-determined number of cells in order increase the number wells in the multi-well device containing the desired number (e.g., a single cell).