The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 05, 2020
Filed:
May. 16, 2017
Applicant:
Cellular Research, Inc., Menlo Park, CA (US);
Inventors:
Glenn Fu, Menlo Park, CA (US);
Eleen Shum, Menlo Park, CA (US);
Assignee:
Cellular Research, Inc., Menlo Park, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6844 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6874 (2013.01);
Abstract
The present disclosure relates to compositions, methods and kits for labeling an internal sequence of a target nucleic acid molecule with molecular barcodes. In some embodiments, the methods comprise intramolecular circulation of a labeled target nucleic acid molecule. Further provided methods for generating sequencing libraries comprising overlapping fragments covering the full length of a target nucleic acid molecule, sequencing the libraries using the methods disclosed herein, and methods of analyzing sequencing results therefrom.