The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 28, 2020

Filed:

Sep. 16, 2013
Applicant:

Dsm Ip Assets B.v., Te Heerlen, NL;

Inventors:

Hugo Streekstra, Amsterdam, NL;

Petrus Joseph Maria Brocken, De Lier, NL;

Assignee:

DSM IP ASSETS B.V., Te Heerlen, NL;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 7/64 (2006.01); A23L 33/12 (2016.01); A23K 20/158 (2016.01); A23D 9/00 (2006.01); A23L 33/115 (2016.01); C12N 1/00 (2006.01); A61Q 19/00 (2006.01); A61K 8/92 (2006.01); A61K 8/67 (2006.01); C11B 1/10 (2006.01); A23D 9/02 (2006.01); A61K 8/99 (2017.01);
U.S. Cl.
CPC ...
C12P 7/6472 (2013.01); A23D 9/00 (2013.01); A23D 9/02 (2013.01); A23K 20/158 (2016.05); A23L 33/115 (2016.08); A23L 33/12 (2016.08); A61K 8/67 (2013.01); A61K 8/925 (2013.01); A61K 8/99 (2013.01); A61Q 19/00 (2013.01); C11B 1/10 (2013.01); C12N 1/005 (2013.01); C12P 7/6427 (2013.01); C12P 7/6481 (2013.01); A61K 2800/85 (2013.01);
Abstract

The present invention provides a process for the production of a microbial oil comprising culturing a micro-organism in a two stage fermentation process where, in a last stage that precedes the end of fermentation, the carbon source is: consumed by the micro-organisms at a rate greater than it is added to the medium; added at a rate #0.30 M carbon/kg medium; or is rate limiting on the growth of the micro-organism. The micro-organisms thus have the carbon source restricted so that they preferentially metabolise fats or lipids other than arachidonic acid (ARA), so increasing the proportion of ARA in the cells. A microbial oil is then recovered from the micro-organism, using hexane as a solvent, that has at least 50% ARA and at least 90% triglycerides.


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