The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 28, 2020

Filed:

May. 18, 2017
Applicants:

National Tsing Hua University, Hsinchu, TW;

Chang Chun Plastics Co., Ltd., Taipei, TW;

Chang Chun Petrochemical Co., Ltd., Taipei, TW;

Inventors:

Roa-Pu Shen, Hsinchu, TW;

Rex C. Wen, Hsinchu, TW;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/16 (2006.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01); C12N 9/12 (2006.01); C12N 9/10 (2006.01); C12N 15/70 (2006.01); C12N 15/52 (2006.01);
U.S. Cl.
CPC ...
C12P 7/16 (2013.01); C12N 9/001 (2013.01); C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/1029 (2013.01); C12N 9/1217 (2013.01); C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/0103 (2013.01); C12Y 101/01027 (2013.01); C12Y 101/01322 (2015.07); C12Y 102/01004 (2013.01); C12Y 103/01086 (2013.01); C12Y 103/05004 (2013.01); C12Y 203/01009 (2013.01); C12Y 207/02001 (2013.01); C12N 2800/101 (2013.01);
Abstract

A butanol expression cassette includes a butanol production related genes and a fermentation regulatory element. The fermentation regulatory element controls the expression of the butanol production related gene and locates upstream of the butanol production related gene. The fermentation regulatory element includes a promoter, a ribosome binding site and a transcription factor binding site of a fermentation gene. A fermentation in which the fermentation regulatory element involves includes an acetic acid fermentation, an alcohol fermentation, a succinic acid fermentation or a lactic acid fermentation, the butanol production related gene is not the fermentation gene or a gene of an upstream product of the fermentation in which the fermentation gene involves. The present invention provides a recombinant plasmid formed by cloning the butanol expression cassettes in the expression vector. The present invention also provides a butanol production related gene expression method to express butanol production related gene by using recombinant plasmid.


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