The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 28, 2020
Filed:
Dec. 18, 2019
New England Biolabs, Inc., Ipswich, MA (US);
Minyong Chen, Ipswich, MA (US);
James C. Samuelson, Newburyport, MA (US);
Ming-Qun Xu, Hamilton, MA (US);
Aihua Zhang, Ipswich, MS (US);
Margaret Heider, Ipswich, MA (US);
Pingfang Liu, Ipswich, MA (US);
New England Biolabs, Inc., Ipswich, MA (US);
Abstract
Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.