The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 21, 2020
Filed:
Nov. 10, 2015
Bgi Shenzhen, Shenzhen, CN;
Bgi Shenzhen Co., Ltd., Shenzhen, CN;
Handong Li, San Jose, CA (US);
Y. Tom Tang, Saratoga, CA (US);
Jing Yu, Shenzhen, CN;
Hui Jiang, Shenzhen, CN;
Wenwei Zhang, Shenzhen, CN;
Guangyi Fan, Shenzhen, CN;
He Zhang, Shenzhen, CN;
Kailong Ma, Shenzhen, CN;
Chunyu Geng, Shenzhen, CN;
BGI Shenzhen, , CN;
BGI Shenzhen Co., Ltd., , CN;
Abstract
Improved single molecule sequencing methods, compositions, and devices, are provided. In a first aspect, the present invention provides a multi-pass method of sequencing a target sequence using nanopore sequencing, the method comprising: i) providing a non-naturally occurring concatemer nucleic acid molecule comprising a plurality of copies of the target sequence; ii) nanopore sequencing at least three copies of the target sequence in the concatemer, thereby obtaining a multi-pass sequence dataset, wherein the multi-pass sequence dataset comprises target sequence datasets for the at least three copies of the target sequence; and iii) using the multi-pass sequence dataset to determine the target sequence.