The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 21, 2020

Filed:

Apr. 17, 2017
Applicant:

Stokes Bio Limited, Shannon Arms, Limerick, IE;

Inventors:

Mark B. Davies, Limerick, IE;

Tara Dalton, Limerick, IE;

Assignee:

STOKES BIO LIMITED, Shannon Arms, IE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6851 (2018.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6851 (2013.01); B01L 3/502784 (2013.01); B01L 7/525 (2013.01); B01L 2300/0838 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0487 (2013.01);
Abstract

Methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a 'droplet-in-oil' technology, may include at least partially segmenting a starting sampled into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point.


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