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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10626381 B1

PDFFull Text
Date of Patent:
Apr. 21, 2020

Filed:

Jun. 23, 2015

Cellulolytic compositions comprising monooxygenase polysaccharide enzymes with improved activity

Applicant:

Abengoa Bioenergía Nuevas Tecnologías, S. A., Seville, ES;

Inventors:

Francisco Manuel Reyes Sosa, Seville, ES;

Bruno Díez García, Seville, ES;

Noelia Valbuena Crespo, Seville, ES;

Antonio Javier Moreno Pérez, Seville, ES;

Dolores Pérez Gómez, Seville, ES;

Ana Isabel Platero Gómez, Seville, ES;

Lucía Martín Pérez, Seville, ES;

Sandra Gavaldá Martín, Seville, ES;

Laura Viñas De La Cruz, Valencia, ES;

Laura Sánchez Zamorano, Seville, ES;

Consolación Álvarez Núñez, Seville, ES;

María de los Angeles Bermúdez Alcántara, Seville, ES;

Javier Rocha Martín, Seville, ES;

Laura Ledesma García, Seville, ES;

Juan Luis Ramos Martín, Seville, ES;

Assignee:

ABENGOA BIOENERGÍA NUEVAS TECNOLOGÍAS, S. A., Seville, ES;

Attorney:

Womble Bond Dickinson (US) LLP

Primary Examiner:

Maryam Monshipouri

Int. Cl.
CPC ...
C12P 7/10 (2006.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01); C12P 19/02 (2006.01); C12P 19/14 (2006.01); C12N 9/24 (2006.01); C12N 9/96 (2006.01); C12N 1/14 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0069 (2013.01); C12N 9/2402 (2013.01); C12N 9/96 (2013.01); C12P 7/10 (2013.01); C12P 19/02 (2013.01); C12P 19/14 (2013.01); C12N 1/14 (2013.01); C12Y 114/13025 (2013.01); Y02E 50/16 (2013.01);
Abstract

The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This improvement is achieved by the addition of a nickel cation to said enzymatic mixtures before and/or during the saccharification step. Thus, the invention provides compositions comprising PMOs, cellulolytic enzymes and a nickel cation, as well as methods for preparing said compositions and methods for producing fermentable sugars and bioproducts, preferably bioethanol, from cellulosic biomass in which said compositions are used.

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