The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 14, 2020
Filed:
Jan. 14, 2016
Applicant:
Kyoto University, Kyoto-shi, Kyoto, JP;
Inventors:
Hirohide Saito, Kyoto, JP;
Seiya Takahashi, Kyoto, JP;
Hidetoshi Sakurai, Kyoto, JP;
Takahiko Sato, Kyoto, JP;
Satoru Takayama, Kyoto, JP;
Assignee:
KYOTO UNIVERSITY, Kyoto, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 15/14 (2006.01); C12N 5/10 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
G01N 15/1434 (2013.01); C12N 5/10 (2013.01); G01N 21/6428 (2013.01); G01N 2015/149 (2013.01); G01N 2021/6439 (2013.01);
Abstract
The present invention aims to provide a method of sorting a skeletal muscle progenitor cell from a cell population containing the skeletal muscle progenitor cell. The above-mentioned problem is solved by providing a step of introducing miRNA-responsive mRNA into a cell population. The miRNA-responsive mRNA contains (i) a nucleic acid having a sequence specifically recognized by miRNA specifically expressed in a skeletal muscle progenitor cell, and (ii) a nucleic acid containing a sequence encoding a marker protein.