The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 14, 2020

Filed:

Nov. 20, 2014
Applicant:

Koninklijke Philips N.v., Eindhoven, NL;

Inventors:

Pieter Jan Van Der Zaag, Waalre, NL;

Anke Pierik, Eindhoven, NL;

Assignee:

KONINKLIJKE PHILIPS N.V., Eindhoven, NL;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/6874 (2018.01); G01N 21/64 (2006.01); B01J 19/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); B01J 19/0046 (2013.01); G01N 21/648 (2013.01); G01N 21/6428 (2013.01); G01N 21/6452 (2013.01); B01J 2219/0059 (2013.01); B01J 2219/00286 (2013.01); B01J 2219/00454 (2013.01); B01J 2219/00495 (2013.01); B01J 2219/00585 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00711 (2013.01); G01N 2021/6439 (2013.01); G01N 2201/061 (2013.01);
Abstract

A device () and a method optically control a chemical reaction in a reaction chamber () holding a reagent fluid (). The chemical reaction includes a nucleic acid sequencing on a wiregrid. Based on strong optical confinement of excitation light () and of cleavage light (), the sequencing reaction can be read-out. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moieties. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked. In order to avoid overheating by cleavage light, the reagent fluid is circulated along the surface of the substrate ().


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