Automated system for the lysis of microorganisms present in a sample, for extraction and for purification of the nucleic acids of said microorganisms for purposes of analysis

Abstract

A device and method for collecting microorganisms from a fluid includes: a reaction module containing beads, fluid admission duct allowing entry into the module, fluid evacuating duct allowing outlet of the fluid passed through the module, retaining beads in the module, at least one channel for admission of reaction liquid, at least one channel for evacuation of the liquid(s), channels for admission and evacuation of the reaction liquid and the ducts for admission and evacuation of the fluid being positioned with: ducts for admission and evacuation of fluid facing and enclosing the module, in order to maximize contact between the fluid and beads, channels for the reaction liquid(s) being positioned respectively at opposite ends of the module, and channels for the reaction liquid(s) positioned in a plane, and ducts for admission and evacuation of fluid positioned along an axis, and in that the axis is roughly perpendicular to the plane.