The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 31, 2020

Filed:

Mar. 07, 2017
Applicant:

Jcr Pharmaceuticals Co., Ltd., Ashiya-shi, Hyogo, JP;

Inventors:

Shinji Kakimoto, Kobe, JP;

Miroslav Matev, Kobe, JP;

Tsuyoshi Fukui, Kobe, JP;

Yukichi Hatano, Kobe, JP;

Junya Tani, Kobe, JP;

Kazutoshi Mihara, Kobe, JP;

Kenichi Takahashi, Kobe, JP;

Atsushi Sugimura, Kobe, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 21/02 (2006.01); C07K 1/16 (2006.01); C12N 15/09 (2006.01); C12N 15/52 (2006.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); C07K 1/16 (2013.01); C12N 15/09 (2013.01); C12N 15/52 (2013.01);
Abstract

Disclosed is a highly efficient method for production of highly pure mutant-type human erythropoietin. The method is for production of mutant-type human erythropoietin, in which a transformed mammalian cell is allowed to produce the mutant-type human erythropoietin, and the supernatant of the culture is subjected to hydrophobic column chromatography, multimodal anion exchange column chromatography, anion exchange column chromatography, phosphate group affinity column chromatography, and gel filtration column chromatography, in this order.


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