The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 31, 2020

Filed:

Jul. 16, 2015
Applicant:

Kyoto University, Kyoto, JP;

Inventors:

Hirohide Saito, Kyoto, JP;

Kei Endo, Kyoto, JP;

Shota Katayama, Kyoto, JP;

Callum Parr, Kyoto, JP;

Assignee:

KYOTO UNIVERSITY, Kyoto, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12N 5/0793 (2010.01); C12N 5/074 (2010.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C12N 5/0619 (2013.01); C12N 5/0696 (2013.01); C12N 2501/385 (2013.01); C12N 2501/65 (2013.01); C12N 2506/45 (2013.01); C12N 2510/00 (2013.01); C12N 2533/50 (2013.01);
Abstract

A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.


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